Journal: Journal of visualized experiments : JoVE

Article Title: Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

doi: 10.3791/59973

Figure Lengend Snippet: Materials

Article Snippet: OmniGrid 100 microarray printer , GeneMachines , , The version of the microarray printer used in this work is no longer commercially available, but the updated similar equipment is the OmniGrid Accent microarray printer from Digilab (Hopkinton, MA), and the same protocol can be carried out with most commercially available microarray printers..

Techniques: Blocking Assay, Imaging, Microarray

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Plasmodium vivax Pre-Erythrocytic-Stage Antigen Discovery: Exploiting Naturally Acquired Humoral Responses

doi: 10.4269/ajtmh.2012.12-0222

Figure Lengend Snippet: Differential recognition of proteins by Fy− and Fy+ donors. Reactivity to proteins on the microarray was compared between 28 Fy− (black bars) and 32 Fy+ (grey bars) and 7 unexposed donors (white bars). Magnitude (A and B) and frequency (C and D) of responses to antigens that are recognized by either Fy− and Fy+ donors or Fy− donors only (A and C) or antigens that are preferentially recognized by Fy+ donors (B and D). Error bars represent standard errors. Positivity was defined as signal intensities 1 SD above the mean of the no-DNA controls spots. *P value between 0.05 and 0.01. **P value between 0.01 and 0.001. ***P value between 0.001 and 0.0001.

Article Snippet: P. vivax protein arrays were printed onto nitrocellulose-coated glass FAST slides (Whatman, Piscataway, NJ) using an OmniGrid Accent microarray printer (DigiLab, Piscataway, NJ); each expressed protein was spotted in triplicate.

Techniques: Microarray